Single B-Cell Screening
With Unprecedented Speed & Resolution


Single B-Cell Screening With Unprecedented Speed & Resolution

High-Resolution Screening in Industry-Leading Timelines

OVERVIEW

The Beacon platform by Berkeley Lights is a disruptive, best-in-industry technology for single B-cell screening. It enables high resolution screening of antibody secreting B-cells with a throughput and timeline that was unimaginable just a few years ago.  Days after tissue collection, the Beacon provides a rich data set about each antibody screened to identify the rare, active, cross-reactive mAbs that are ideal for clinical development. Those antibodies are then sequenced and expressed rapidly to advance them to downstream assays.

The Beacon platform is a perfect combination with our ability to generate immune responses in-vivo against difficult-to-target epitopes and our expertise in downstream characterization.

By screening mAbs in for the proper functional activity and cross-reactivity upfront the process is much more efficient than the age-old method of developing and expressing thousands of mAbs to identify the few with relevant function. This results in better mAbs, delivered faster and with less wasted resources.

Beacon image

ADVANTAGES

  • High resolution screening in days after tissue collection including:
    • Cross-reactivity screening on multiple cell lines and recombinant reagents
    • Activity assays
    • Affinity screening
    • Developability assessment
  • Exceptionally rapid timelines – saving months of time
  • Delivery of antibody sequences and associated rich screening data early
  • Ability to identify rare, therapeutically relevant antibodies directly from B-cells
  • Take advantage of the increased diversity of our DiverisimAbTM Platform
  • Human mAb discovery available using Trianni, Harbour and other humanized mouse platforms

WORKFLOW EXAMPLE

antibody_whitebgrd

1. Immunizations - 3-5 Weeks

Humanized mice (Trianni, Harbour, others) as well as our DiversimAb and K/O mice available. Our team has experience and IP for generating an immune response against even the hardest of targets.

antibody_whitebgrd

3. Antibody Sequencing - 1-2 Weeks

Optimized protocols and primers enable rapid, efficient sequence capture from single B-cells exported from the Beacon

antibody_whitebgrd

2. Single B-Cell Screening on the Beacon - 1 Week

Activity assays, on-cell binding, and cross-reactivity data the same day as tissue collections allow us to advance only ideal antibodies to downstream expression.

antibody_whitebgrd

4. Expression, Advanced Analysis - 4-7 Weeks

Flow cytometry (iQue), Octet, ELISA and Carterra SPR platforms are available to identify ideal mAbs rapidly from small-scale purifications.

WANT TO LEARN MORE?

Get in touch with our scientists to discuss your project!

We like to meet in person with our Boston clients. We can setup a Zoom to connect if you're further away.